Tutorial

  1. Download the sample input file
$ curl -O http://paoyang.ipmb.sinica.edu.tw/public_data/methgo_demo.tar.gz
$ tar xvfz methgo_demo.tar.gz
$ cd methgo_demo/data
  1. Run COV module:
$ methgo cov genome.fa demo.CGmap
_images/demo.cov.png
  1. Run MET module:
$ methgo met genes.gtf genome.fa demo.CGmap
_images/demo.bulk.hist.png _images/demo.bulk.mean.png _images/demo.feature.CG.png _images/demo.feature.CHG.png _images/demo.feature.CHH.png _images/demo.genomewide.png
  1. Run TXN module:
$ methgo txn -t methgo/scripts/txn/tair10_txn -l ATHB1_binding_site_motif,CCA1_binding_site_motif -c demo.CGmap
_images/demo.CG.txn.png _images/demo.CHG.txn.png _images/demo.CHH.txn.png
  1. Run SNP module:
$ methgo snp -g genome.fa demo.sorted.bam
  1. Run CNV module:
$ methgo cnv genome.fa.fai demo.sorted.bam
_images/demo.sorted.cnv.png